Applications of random amplified polymorphic DNA (RAPD) in molecular ecology

被引：700
|
作者：
Hadrys, H. [1 ]
Balick, M. [2 ]
Schierwater, B. [1 ]
机构：
[1] Yale Univ, Dept Biol, New Haven, CT 06511 USA
[2] New York Bot Garden, Bronx, NY 10458 USA
来源：
MOLECULAR ECOLOGY | 1992年 / 1卷 / 01期
关键词：
DNA fingerprinting; DNA probes; kinship analysis; paternity determination; RAPD; taxonomic identifications;
D O I：
10.1111/j.1365-294X.1992.tb00155.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Molecular genetic markers have been developed into powerful tools to analyse genetic relationships and genetic diversity. As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes. Main advantages of the RAPD technology include (i) suitability for work on anonymous genomes, (ii) applicability to problems where only limited quantities of DNA are available, (iii) efficiency and low expense.
引用
收藏
页码：55 / 63
页数：9
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