CHARACTERIZATION BY 2D-NMR AND CHEMICAL-PROPERTIES OF (20Z)-3-METHOXY-17-[2-(TRIPHENYLSTANNYL)VINYL]ESTRA-1,3,5(10)-TRIEN-17-BETA-OL

The complete solution structure and conformation of the title compound were determined by 2D proton detected H-1-C-13 HMQC-RELAY and 2D-NOESY build-up experiments. The assignment of the low field resonances of the quaternary carbon atoms was achieved by 1D-C-13 NMR with selective decoupling of the high field protons. Proton detected H-1-Sn-119 HMBC 1D and 2D NMR spectroscopy enables the observation of a very weak coupling between the hydroxy proton of the steroid moiety and the Sn-119 nucleus. These proton detected experiments as well as C-13 and Sn-119 SIMPLE NMR provide evidence for a HO-->Sn coordination to occur in solution.