IDENTIFICATION AND LOCALIZATION OF APHID LETHAL PARALYSIS VIRUS-PARTICLES IN THIN TISSUE-SECTIONS OF THE RHOPALOSIPHUM-PADI APHID BY INSITU NUCLEIC-ACID HYBRIDIZATION

被引：16
|
作者：
HATFILL, SJ [1 ]
WILLIAMSON, C [1 ]
KIRBY, R [1 ]
VONWECHMAR, MB [1 ]
机构：
[1] UNIV CAPE TOWN,DEPT MICROBIOL,RONDEBOSCH 7700,SOUTH AFRICA
来源：
JOURNAL OF INVERTEBRATE PATHOLOGY | 1990年 / 55卷 / 02期
关键词：
aphid lethal paralysis virus; detection of pathogen; Family Aphididae; family Picornaviridae; in situ nucleic acid hybridization; subfamily Aphidinae; subtribe Rhopalisiphina; tribe Aphidini;
D O I：
10.1016/0022-2011(90)90062-B
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
Nucleic acid in situ hybridization using 32P-end-labeled viral genomic RNA was used as an alternative to electron microscopy for the identification and localization of virus particles in whole-body thin-tissue sections of aphids. Actively replicating aphid lethal paralysis virus (ALPV), a picorna-like virus of aphids, was detected in the stomach and intestinal epithelium of infected Rhopalosiphum padi aphids. ALPV was also detected throughout the protocerebrum of aphids in the advanced stages of infection. Actively replicating virions were also detected in developing embryos indicating that transovarial transmission of an active maternal ALPV infection does occur. © 1990.
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页码：265 / 271
页数：7
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