ASPARTAME PRECURSOR SYNTHESIS IN WATER MISCIBLE COSOLVENT CATALYZED BY THERMOLYSIN

被引：7
|
作者：
LEE, KH
LEE, PM
SIAW, YS
MORIHARA, K
机构：
[1] INDIANA UNIV,DEPT CHEM,SHAH ALAM CAMPUS MALAYSIA,ITM MUCIA,SECTION 17,SHAH ALAM 40000,MALAYSIA
[2] UNIV PERTANIAN MALAYSIA,FAC FOOD SCI & BIOTECHNOL,DEPT BIOTECHNOL,SERDANG 43400,MALAYSIA
[3] UNIV E ASIA,INST APPL LIFE SCI,YAMAGUCHI 751,JAPAN
来源：
BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 12期
关键词：
D O I：
10.1007/BF01027021
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 muM in 25 % methanol can catalyse the synthetic reaction. The optimum methanol compositions at 4-degrees-C and 37-degrees-C were 50 % and 25 % respectively where an increase in peptide yield of 85 % was obtained for both conditions as compared to that in water.
引用
收藏
页码：1159 / 1164
页数：6
相关论文
共 50 条
  • [1] Mathematical approach to thermolysin-catalyzed synthesis of aspartame precursor
    Murakami, Y
    Hirata, M
    Hirata, A
    JOURNAL OF FERMENTATION AND BIOENGINEERING, 1996, 82 (03): : 246 - 252
  • [2] Papain-catalyzed synthesis of aspartame precursor: A comparison with thermolysin
    Nakaoka, H
    Miyajima, Y
    Morihara, K
    JOURNAL OF FERMENTATION AND BIOENGINEERING, 1998, 85 (01): : 43 - 47
  • [3] IMMOBILIZED THERMOLYSIN AND SYNTHESIS OF PRECURSOR OF ASPARTAME
    ZHOU, QZ
    HUANG, Z
    INDIAN JOURNAL OF CHEMISTRY SECTION B-ORGANIC CHEMISTRY INCLUDING MEDICINAL CHEMISTRY, 1993, 32 (01): : 35 - 39
  • [4] New method for analysis of enzymatic reactions: Thermolysin-catalyzed synthesis of aspartame precursor
    Murakami, Y
    Hirata, A
    JOURNAL OF FERMENTATION AND BIOENGINEERING, 1997, 83 (01): : 38 - 42
  • [5] SYNTHESIS OF AN ASPARTAME PRECURSOR BY IMMOBILIZED THERMOLYSIN IN AN ORGANIC-SOLVENT
    OYAMA, K
    NISHIMURA, S
    NONAKA, Y
    KIHARA, KI
    HASHIMOTO, T
    JOURNAL OF ORGANIC CHEMISTRY, 1981, 46 (25): : 5241 - 5242
  • [6] SYNTHESIS OF ASPARTAME PRECURSOR BY SOLID THERMOLYSIN IN ORGANIC-SOLVENT
    OOSHIMA, H
    MORI, H
    HARANO, Y
    BIOTECHNOLOGY LETTERS, 1985, 7 (11) : 789 - 792
  • [7] SYNTHESIS OF ASPARTAME PRECURSOR WITH AN IMMOBILIZED THERMOLYSIN IN TERT-AMYL ALCOHOL
    NAGAYASU, T
    MIYANAGA, M
    TANAKA, T
    SAKIYAMA, T
    NAKANISHI, K
    BIOTECHNOLOGY AND BIOENGINEERING, 1994, 43 (11) : 1118 - 1123
  • [8] SYNTHESIS OF ASPARTAME PRECURSOR WITH AN IMMOBILIZED THERMOLYSIN IN MIXED ORGANIC-SOLVENTS
    MIYANAGA, M
    TANAKA, T
    SAKIYAMA, T
    NAKANISHI, K
    BIOTECHNOLOGY AND BIOENGINEERING, 1995, 46 (06) : 631 - 635
  • [9] LONG-TERM CONTINUOUS SYNTHESIS OF ASPARTAME PRECURSOR IN A COLUMN REACTOR WITH AN IMMOBILIZED THERMOLYSIN
    NAKANISHI, K
    TAKEUCHI, A
    MATSUNO, R
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1990, 32 (06) : 633 - 636
  • [10] PAPAIN-CATALYZED SYNTHESIS OF ASPARTAME PRECURSOR IN BIPHASIC SYSTEM
    CHEN, ST
    WANG, KT
    JOURNAL OF ORGANIC CHEMISTRY, 1988, 53 (19): : 4589 - 4590
12345