PURIFICATION AND SOME PROPERTIES OF SQUALENE-2,3-EPOXIDE-LANOSTEROL CYCLASE FROM RAT-LIVER

被引：0
|
作者：
KUSANO, M [1 ]
ABE, I [1 ]
SANKAWA, U [1 ]
EBIZUKA, Y [1 ]
机构：
[1] UNIV TOKYO, FAC PHARMACEUT SCI, 7-3-1 HONGO, BUNKYO KU, TOKYO 113, JAPAN
来源：
CHEMICAL & PHARMACEUTICAL BULLETIN | 1991年 / 39卷 / 01期
关键词：
SQUALENE-2,3-EPOXIDE; LANOSTEROL; CYCLASE; RAT LIVER;
D O I：
暂无
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Squalene-2,3-epoxide:lanosterol cyclase was purified from rat liver in five steps as a soluble and homogeneous protein. The purified enzyme showed a single band on SDS-polyacrylamide gel electrophoresis with a molecular weight of 75 kD. In its native state it behaved as a homo-dimer. The isoelectric point of 5.5 and the apparent Km value for (3S)-squalene-epoxide of 55-mu-M were estimated for the cyclase.
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页码：239 / 241
页数：3
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