ENDOCYTOSIS OF THE TCR/CD3 COMPLEX AND THE CLASS-I MAJOR HISTOCOMPATIBILITY COMPLEX IN A HUMAN T-CELL LINE

被引：8
|
作者：
YAMANE, Y
PEREZ, M
EDELSON, R
AGOSTINO, N
PERNIS, B
机构：
[1] COLUMBIA UNIV COLL PHYS & SURG,DEPT MICROBIOL,701 W 168TH ST,NEW YORK,NY 10032
[2] YALE UNIV,DEPT DERMATOL,NEW HAVEN,CT 06520
来源：
CELLULAR IMMUNOLOGY | 1991年 / 136卷 / 02期
关键词：
D O I：
10.1016/0008-8749(91)90370-Q
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We investigated the expression of the T cell receptor (TCR)/CD3 complex on a CD4-positive human T cell lymphoma cell line treated with phorbol myristate acetate (PMA) and/or Ca2+ ionophore using fluorescence flow cytometry and fluorescence microscopic analysis. PMA induced a significant decrease in the expression of the CD3 complex on the cell membranes. Fluorescence microscopy confirmed that the down regulation is due to internalization of the antigens. Ca2+ ionophore treatment had no effect on the internalization of the CD3 complex. Double staining revealed that the vesicles containing the internalized CD3 complex and those containing intracytoplasmic class I major histocompatibility complex antigen had similar distribution in the PMA-stimulated cells, implying coexistence of these two antigens in a cytoplasmic perinuclear distribution. © 1991.
引用
收藏
页码：496 / 503
页数：8
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