CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE RECEPTORS IN RETINA

被引：16
|
作者：
SWEDLUND, AP
ROSENZWEIG, SA
机构：
[1] YALE UNIV,SCH MED,DEPT OPHTHALMOL & VISUAL SCI,330 CEDAR ST,POB 3333,NEW HAVEN,CT 06510
[2] YALE UNIV,SCH MED,DEPT INTERNAL MED,NEW HAVEN,CT 06510
[3] YALE UNIV,SCH MED,DEPT CELL BIOL,NEW HAVEN,CT 06510
来源：
EXPERIMENTAL EYE RESEARCH | 1990年 / 51卷 / 03期
关键词：
affinity labeling; endoglycosidase F; gut Hormone; neuronal receptor subtype; neuropeptide; neuropeptide receptor; retina; VIP; VIP Receptor;
D O I：
10.1016/0014-4835(90)90028-S
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Vasoactive intestinal peptide (VIP) is a neuropeptide having a wide range of effects on a large number of tissues. To gain insight into the role VIP plays in retinal function, VIP receptors in bovine retinal membranes were analyzed in competition binding assays and by affinity labeling studies and compared to VIP receptors in rat liver membranes. In both membrane preparations, high affinity VIP binding sites (KD ∼ 1 nm) were detected. Secretin and glucagon, each having close structural homology to VIP, were found to have negligible effects on [125I]VIP binding in retina. In contrast, secretin (KD = 70 nm) was modestly effective in inhibiting [125I]VIP binding to rat liver membranes. Affinity labeling analysis revealed a VIP binding site of 59 kDa in both bovine retinal and rat liver membranes. Digestion of affinity-labeled receptor proteins with endoglycosidase F generated final cleavage products of approx. 45 kDa for both receptors. These results indicate that the retina expresses a high affinity, highly selective VIP receptor thereby supporting a specific function for VIP in this tissue. © 1990.
引用
收藏
页码：317 / 323
页数：7
相关论文
共 50 条
  • [1] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE RECEPTORS IN HUMAN LIVER
    RODRIGUEZHENCHE, N
    RODRIGUEZPENA, MS
    GUIJARRO, LG
    PRIETO, JC
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1994, 1221 (02): : 193 - 198
  • [2] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE RECEPTORS IN RABBIT CILIARY PROCESSES
    HORIO, B
    LAW, NM
    ROSENZWEIG, SA
    [J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1995, 36 (01) : 192 - 199
  • [3] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE (VIP) RECEPTORS IN MAMMALIAN LUNG
    DICKINSON, KEJ
    SCHACHTER, M
    MILES, CMM
    SEVER, PS
    [J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1986, 133 (04): : A141 - A141
  • [4] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE (VIP) RECEPTORS IN MAMMALIAN LUNG
    DICKINSON, KEJ
    SCHACHTER, M
    MILES, CMM
    COY, DH
    SEVER, PS
    [J]. PEPTIDES, 1986, 7 (05) : 791 - 800
  • [5] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE RECEPTORS IN CANINE LIVER MEMBRANES
    KISO, T
    ITO, S
    OHTA, T
    ASANO, T
    NAKAZATO, Y
    [J]. BIOCHEMICAL PHARMACOLOGY, 1994, 47 (02) : 241 - 245
  • [6] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE RECEPTORS ON RAT ALVEOLAR MACROPHAGES
    SAKAKIBARA, H
    SHIMA, K
    SAID, SI
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 267 (03): : L256 - L262
  • [7] CHARACTERIZATION OF RECEPTORS FOR VASOACTIVE-INTESTINAL-PEPTIDE SOLUBILIZED FROM THE LUNG
    PAUL, S
    SAID, SI
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1987, 262 (01) : 158 - 162
  • [8] LOCALIZATION AND CHARACTERIZATION OF FUNCTIONAL VASOACTIVE-INTESTINAL-PEPTIDE RECEPTORS IN FELINE KIDNEY
    GRIFFITHS, NM
    SIMMONS, NL
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1990, 416 (1-2): : 80 - 87
  • [9] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE RECEPTORS IN RAT SEMINAL-VESICLE
    GUIJARRO, LG
    RODRIGUEZPENA, MS
    PRIETO, JC
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (02): : E286 - E291
  • [10] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE RECEPTORS ON RAT-LIVER MICROSOMES
    NGUYEN, TD
    HEINTZ, GG
    WOLFE, MS
    [J]. HEPATOLOGY, 1991, 14 (04) : A186 - A186
12345