Antioxidant Activity of the Leaves and Stems in Different Growth Stage of Methanol Extract From Datura stramonium Plant from Sistan and Baluchestan

Stramonium is a species of Datura genus and were grown in the botanic garden of the University of Sistan and Baluchestan and were studied the collected samples in flowering period. The methanol extracts of the samples were extracted by Soxhlet apparatus and were concentrated in a rotary. Antioxidant activity of the samples was measured by using DPPH and FRAPS methods. Methanol extracts as compared with synthetic antioxidant BHT is better than other extracts showed good effect. DPPH method showed highest antioxidant activity for flowers and roots in after flowering period and for leaves in during flowering period (IC50=48.95 and 45.84 and 35.78 respectively) compared with the amount for leaves and stems in after flowering period and for stems in before flowering period (IC50=31.50 and 6.59 and 11.38, respectively). FRAP assay showed highest antioxidant activity for flowers and roots in after flowering period and for leaves in during flowering period (mMFe+2/sample=60.75 and 40.78 and 56.92 respectively) compared with the amount for leaves and stems in after flowering period and for stems in before flowering period (mMFe+2/sample=30.54 and 19.61 and 30.50, respectively.