THE LAMELLAR TO HEXAGONAL PHASE-TRANSITION IN PHOSPHATIDYLETHANOLAMINE LIPOSOMES - A FLUORESCENCE ANISOTROPY STUDY

被引:8
|
作者
NIEVA, JL [1 ]
CASTRESANA, J [1 ]
ALONSO, A [1 ]
机构
[1] UNIV BASQUE COUNTRY,FAC SCI,DEPT BIOCHEM,POB 644,E-48080 BILBAO,SPAIN
关键词
D O I
10.1016/0006-291X(90)91126-D
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The steady-state anisotropy of trimethylammonium diphenylhexatriene fluorescence has been used to monitor the thermotropic lamellar to Hii hexagonal phase transition in an unsaturated phosphatidylethanolamine. The transition is observed in lipid aggregates when they are heated above the transition temperature Th, as well as in diluted liposomes after aggregation above Th. Changes in fluorescence anisotropy are not observed with Ca++-induced fusion of phosphatidylserine vesicles, a process not involving hexagonal phase formation. © 1990.
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页码:987 / 992
页数:6
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