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CALCIUM ION-REGULATED PHOSPHOLIPASE-C ACTIVITY IN BOVINE ROD OUTER SEGMENTS

被引：18
|
作者：
PANFOLI, I
MORELLI, A
PEPE, I
机构：
[1] CNR,IST CIBERNET & BIOFIS,I-16146 GENOA,ITALY
[2] UNIV GENOA,IST POLICATTEDRA CHIM BIOL,V BENEDETTO XV 1,I-16132 GENOA,ITALY
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 173卷 / 01期
关键词：
D O I：
10.1016/S0006-291X(05)81054-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bovine retinal rod outer segment membranes are enriched in a phosphoinositide-specific phosphodiesterase (phospholipase C) activity strictly modulated by free calcium ion concentration. The enzyme(s) was highly active on phosphatidylinositol 4,5-bisphosphate: maximal hydrolysis rate was attained at 10-5M Ca2+ and accounted for 91 ± 4 nmoles hydrolyzed/ min/ mg of protein. The results support the notion that in vivo the enzyme(s) is regulated so as to conform to the phototransduction events. © 1990 Academic Press, Inc.
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收藏
页码：283 / 288
页数：6
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