SEPARATION OF PORPHYRINS USING A GAMMA-CYCLODEXTRIN STATIONARY-PHASE

被引:3
|
作者
WU, WH
STALCUP, A
机构
[1] Department of Chemistry, 2545 The Mall, University of Hawaii at Manoa, Honolulu
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 05期
基金
美国国家卫生研究院;
关键词
D O I
10.1080/10826079408013389
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An isocratic separation of five porphyrin carboxylic acids using conventional mobile phase conditions was achieved on a commercially available gamma-cyclodextrin bonded phase within 30 minutes. The effects of pH, ionic strength, organic modifier as well as the retention and separation mechanisms are discussed. The direct determination of porphyrin levels in blood serum is also discussed.
引用
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页码:1111 / 1124
页数:14
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