A NOVEL ROLE FOR DOPAMINE - INHIBITION OF MUSCARINIC CHOLINERGIC-STIMULATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT-BRAIN CORTICAL MEMBRANES

被引：23
|
作者：
WALLACE, MA
CLARO, E
机构：
[1] University of Tennessee, Memphis, Department of Biochemistry, Memphis
来源：
NEUROSCIENCE LETTERS | 1990年 / 110卷 / 1-2期
关键词：
Brain cortex; Carbachol; Dopamine; Muscarinic; Phosphoinositide; Phospholipase C;
D O I：
10.1016/0304-3940(90)90804-I
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Dopamine inhibited phosphoinositide breakdown as stimulated by carbachol in rat brain cortical membranes. The IC50 value was 14 ± 2 μM for dopamine's inhibition of phosphatidylinositol hydrolysis as stimulated by 1 mM carbachol. The inhibition was found at low (0.1 μM), but not high (> 0.3 μM), concentrations of the non-hydrolyzable guanine nucleotide analog, GTPγS. Pharmacological characterization of the response indicated that the dopamine effects were mediated by D1 receptors. The assay conditions precluded any involvement of cyclic-AMP as a mediator of the dopamine response, and thus, a novel role is proposed for dopamine in cortex working through D1 receptors to inhibit phosphoinositide degradation. © 1990.
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页码：155 / 161
页数：7
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