CA-2+ UPTAKE DURING CAPACITATION OF MOUSE SPERMATOZOA AND THE EFFECT OF AN ANION TRANSPORT INHIBITOR ON CA-2+ UPTAKE

被引：44
|
作者：
RUKNUDIN, A [1 ]
SILVER, IA [1 ]
机构：
[1] UNIV BRISTOL, SCH MED, DEPT PATHOL, BRISTOL BS8 1TH, AVON, ENGLAND
来源：
MOLECULAR REPRODUCTION AND DEVELOPMENT | 1990年 / 26卷 / 01期
关键词：
Bicarbonate ions; Ca[!sup]2+[!/sup] microelectrode; Fertilization; Mammalian spermatozoa;
D O I：
10.1002/mrd.1080260110
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
With a specially constructed chamber, Ca2+ uptake by mouse spermatozoa was monitored continuously during capacitation and the acrosome reaction. It was shown, using calcium ion‐selective microelectrodes, that there was an initial uptake of Ca2+ by spermatozoa undergoing capacitation. Such net transport was also promoted by the divalent cation ionophores A23187 or ionomycin. An anion inhibitor, SITS, produced dose‐dependent inhibition of Ca2+ uptake. This inhibitor reduced the incidence of capacitation as revealed by a reduction in the B pattern by chlortetracycline (CTC) assay and thus inhibited fertilization, suggesting that anions are involved in calcium uptake in mouse spermatozoa. Copyright © 1990 Wiley‐Liss, Inc.
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页码：63 / 68
页数：6
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