HUMAN BLOOD GROUP-B-ACTIVE GANGLIO-GLYCOSPHINGOLIPID IN RAT GLIOMA

被引：0
|
作者：
SUETAKE, K
GASA, S
TAKI, T
CHIBA, M
YAMAKI, T
IBAYASHI, Y
HASHI, K
机构：
[1] SAPPORO MED UNIV,SCH CHEM,DEPT NEUROSURG,SAPPORO,JAPAN
[2] TOKYO MED & DENT UNIV,DEPT BIOCHEM,TOKYO 113,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1170卷 / 01期
关键词：
GLYCOSPHINGOLIPID; GANGLIOGLYCOLIPID; B-ACTIVE LIPID; GLIOMA; (RAT);
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A human blood group B-active glycosphingolipid, belonging to the ganglio-series, was isolated from rat glioma cell line RG2 subcutaneous isografts. The oligosaccharide structure of the glycosphingolipid was completely characterized as Galalpha1-3(Fucaalpha1-2)Galbeta1-3GalNAcbeta1-4Galbeta1-4Glcbeta1-1' ceramide by NMR spectrometry, negative fast atom bombardment-mass spectrometry, sequential degradation by glycosidases and methylation analysis. Human blood group B antigenicity and the activity of this glycosphingolipid were confirmed by immunostaining on thin-layer chromatography and the inhibition of hemagglutination, respectively. Although the lipid has been detected in rat granuloma, bone marrow cells, spleen, thymus, ascites hepatoma cells and gastric mucosa, this is the first report of the occurrence of the B-active lipid in glioma.
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页码：25 / 31
页数：7
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