LIPID HYDROPEROXIDE MEASUREMENT BY OXIDATION OF FE2+ IN THE PRESENCE OF XYLENOL ORANGE - COMPARISON WITH THE TBA ASSAY AND AN IODOMETRIC METHOD

被引：545
|
作者：
JIANG, ZY [1 ]
WOOLLARD, ACS [1 ]
WOLFF, SP [1 ]
机构：
[1] UNIV COLL & MIDDLESEX SCH MED,DEPT CLIN PHARMACOL,TOXICOL LAB,5 UNIV ST,LONDON WC1E 6JJ,ENGLAND
来源：
LIPIDS | 1991年 / 26卷 / 10期
关键词：
D O I：
10.1007/BF02536169
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Study of the role of hydroperoxides and lipid peroxidation in disease requires simple and sensitive methods for direct hydroperoxide measurement. We report on a technique for measuring hydroperoxide which relies upon the rapid hydroperoxide-mediated oxidation of Fe2+ under acidic conditions. Fe3+ forms a chromophore with xylenol orange which absorbs strongly at 560 rum, yielding an apparent E560 (for H2O2, butyl hydroperoxide and cumene hydroperoxide) of 4.3 X 10(4) M-1 cm-1. The assay was validated in a study of liposomal lipid peroxidation and shown to give results comparable with those obtained by an iodometric method or by measuring conjugated dienes. The assay involving thiobarbituric acid, by comparison, underestimates lipid peroxidation and does not measure hydroperoxide per se.
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页码：853 / 856
页数：4
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