ESTABLISHMENT AND CHARACTERISTICS OF A CELL-LINE FROM FETAL BOVINE THYROID (FBTY)

被引：0
|
作者：
FISCHER, O
MACHATKOVA, M
PECUCHOVA, L
ZENDULKOVA, D
HORINOVA, Z
机构：
来源：
ACTA VETERINARIA BRNO | 1994年 / 63卷 / 02期
关键词：
MITOSIS; INDIRECT IMMUNOFLUORESCENCE; BOVINE ADENOVIRUS;
D O I：
10.2754/avb199463020081
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Characteristics of a cell line from fetal bovine thyroid were tested in the 150th passage. The line was adapted to growth in the minimal essential medium supplemented with 10% bovine fetal serum and it had an epithelioid morphology. After seeding of 1 x 10(4) cells/ml, the culture density was higher than 1 x 10(6) cells/ml on day 6. The FBTY line was heteroploid. The average number of chromosomes was 48.8 +/- 10.0 and only 1% of mitoses had a diploid number of chromosomes (2n = 60). Species specificity (Bos taurus) was confirmed by indirect immunofluorescence. Besides pronounced fluorescence with rabbit antisera against bovine proteins, weak reactions with antisera against ovine, caprine and swine proteins were observed. The FBTY line was susceptible to two strains of parainfluenza 3 virus, six strains of infectious bovine rhinotracheitis virus and bovine adenoviruses of the serovars 1, 2, 3, and 8.
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页码：81 / 87
页数：7
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