COMPARISON OF TECHNIQUES FOR H-1-DETECTED HETERONUCLEAR H-1-N-15 SPECTROSCOPY

It has previously been demonstrated that 1H-detected heteronuclear 1H15N correlation techniques increase the number of proteins that can be usefully studied by NMR. All previous studies of this type have utilized 1H15N heteronuclear multiple-quantum coherence to encode 15N chemical shift. In this paper we show that 15N single-quantum coherence has several significant advantages including substantially better 15N resolution (and a reduction in the transverse relaxation rate of coherence evolving during the t1 evolution period). Experiments based upon the two techniques are demonstrated and compared using a sample of the c subunit of the Escherichia coli F1-F0 ATP-synthase. © 1990.