EVALUATION OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DETECTION OF ANTIBODIES TO VESICULAR STOMATITIS-VIRUS IN CATTLE IN AN ENZOOTIC REGION OF MEXICO

被引：0
|
作者：
DEANDA, JH
SALMAN, MD
WEBB, PA
KEEFE, TJ
AREVALO, AA
MASON, J
机构：
[1] COLORADO STATE UNIV, COLL VET MED & BIOMED SCI, DEPT ENVIRONM HLTH, FT COLLINS, CO 80523 USA
[2] CTR DIS CONTROL, DIV VECTOR BORNE DIS, FT COLLINS, CO 80522 USA
[3] INST NACL INVEST FORESTALES & AGROPECUARIAS, CAMPO EXPTL PECUARIO, VERACRUZ 95600, MEXICO
[4] USDA, APHIS, FOOT & MOUTH DIS PROGRAM MEXICO, LAREDO, TX 78040 USA
[5] UNIV AUTONOMA BAJA CALIF, INST INVEST & CIENCIAS VET, BAJA CALIFORNIA, MEXICO
来源：
AMERICAN JOURNAL OF VETERINARY RESEARCH | 1992年 / 53卷 / 04期
关键词：
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
An ELISA was compared with the plaque-reduction serum neutralization (PRSN) test, for detection of vesicular stomatitis virus (VSV) antibodies in cattle in a vesicular stomatitis enzootic region of Mexico. A total of 325 bovine serum samples were screened for VSV antibodies. The PRSN test was performed, using Vero cells. The ELISA contained gradient-purified VSV Indiana (Lab strain) and VSV New Jersey (Hazelhurst) as the antigens. Regression analysis and weighted kappa statistic were used to estimate measures of agreement between the 2 assays for detection of VSV antibodies. The ELISA method proved useful for serodiagnosis of vesicular stomatitis. The ELISA and PRSN test results were highly correlated for detection of VSV antibodies.
引用
收藏
页码：440 / 443
页数：4
相关论文
共 50 条
12345