PREPARATION AND CHARACTERIZATION OF A MURINE MONOCLONAL-ANTIBODY (MDR3M) REACTIVE WITH MDR3 GENE-PRODUCT

被引：5
|
作者：
SUGAWARA, I [1 ]
HIRATA, A [1 ]
UEDA, K [1 ]
ITOYAMA, S [1 ]
机构：
[1] KYOTO UNIV,DEPT AGR,SAKYO KU,KYOTO 606,JAPAN
来源：
JAPANESE JOURNAL OF CANCER RESEARCH | 1992年 / 83卷 / 08期
关键词：
MDR3 GENE PRODUCT; MONOCLONAL ANTIBODY; MDR3; PEPTIDE;
D O I：
10.1111/j.1349-7006.1992.tb01982.x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3-specific peptide (H2N-12WRPTSAEGDFELGISSKQKRKKTKTVKMI41G-COOH) and hybridizing the primed mouse splenic B cells with X63-Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did not cross-react with mdr1 gene product. This monoclonal antibody may be useful for analyzing the role of mdr3 gene product in cells and tissues.
引用
收藏
页码：795 / 797
页数：3
相关论文
共 50 条
  • [1] MDR3 gene mutations and cholesterol cholelithiasis
    Rosmorduc, O
    Hermelin, B
    Poupon, R
    BILE ACIDS: FROM GENOMICS TO DISEASE AND THERAPY, 2003, 129 : 193 - 198
  • [2] MDR3 deficiency: Progressive familial intrahepatic cholestasis (PFIC) due to MDR3 gene mutations.
    Jacquemin, E
    de Vree, JML
    Cresteil, D
    Dumont, M
    Bernard, O
    Hadchouel, M
    Elferink, RPJ
    HEPATOLOGY, 1998, 28 (04) : 316A - 316A
  • [3] A new monoclonal antibody that specifically recognises the MDR-3-encoded gene product
    Larkin, A
    Moran, E
    Alexander, D
    Doherty, G
    Connolly, L
    Kennedy, SM
    Clynes, M
    INTERNATIONAL JOURNAL OF CANCER, 1999, 80 (02) : 265 - 271
  • [4] STRUCTURE OF THE HUMAN MDR3 GENE AND PHYSICAL MAPPING OF THE HUMAN MDR LOCUS
    LINCKE, CR
    SMIT, JJM
    VANDERVELDEKOERTS, T
    BORST, P
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1991, 266 (08) : 5303 - 5310
  • [5] MONOCLONAL-ANTIBODY AGAINST AN IR GENE-PRODUCT
    LERNER, EA
    MATIS, LA
    JANEWAY, CA
    JONES, PP
    SCHWARTZ, RH
    MURPHY, DB
    JOURNAL OF EXPERIMENTAL MEDICINE, 1980, 152 (04): : 1085 - 1101
  • [6] Functional characterization of genetic variations in the MDR3 promoter
    Jang, Geun Hye
    Kim, Tae Hee
    Choe, Yunseo
    Ham, Ahrong
    Choi, Ji Ha
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 430 (04) : 1312 - 1318
  • [7] Human MDR1 and MDR3 gene expression in the liver with obstructive jaundice
    Nozawa, S
    Iyazaki, MM
    Nakagawa, KN
    Ambiru, S
    Shimizu, H
    Kato, A
    Yoshitomi, H
    Kawaki, J
    Nakajima, N
    GASTROENTEROLOGY, 1997, 112 (04) : A1349 - A1349
  • [8] Expression of the mdr1 and mdr3 gene products in acute and chronic leukemias
    Arai, Y
    Masuda, M
    Sugawara, I
    Arai, T
    Motoji, T
    Tsuruo, T
    Oshimi, K
    Mizoguchi, H
    LEUKEMIA RESEARCH, 1997, 21 (04) : 313 - 319
  • [9] CHARACTERIZATION OF THE PROMOTER REGION OF THE HUMAN MDR3 P-GLYCOPROTEIN GENE
    SMIT, JJM
    MOL, CAAM
    VANDEEMTER, L
    WAGENAAR, E
    SCHINKEL, AH
    BORST, P
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1995, 1261 (01): : 44 - 56
  • [10] ABCB4/MDR3 gene mutations and cholangiocarcinomas
    Tougeron, David
    Fotsing, Ginette
    Barbu, Veronique
    Beauchant, Michel
    JOURNAL OF HEPATOLOGY, 2012, 57 (02) : 467 - 468
12345