SEPARATION OF SELENIUM ANALOGS OF SULFUR-CONTAINING AMINO-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND HIGH-RESOLUTION GAS-CHROMATOGRAPHY

The historically conditioned adaptation of living organisms to chemically corresponding elements is influenced in nature by anthropogenic activities in many regions, the selenium-sulphur pair being one example of such a case. The separation of selenomethionine, selenoethionine and selenocystine was studied by HPLC and high-resolution GC. Ion-exchange chromatography followed by temperature-programmed GC gives the possibility of the analytical separation of trace amounts of selenomethionine in a complex mixture of common amino acids. Diastereoisomers of selenocystine were identified by HPLC in the AccQ-Tag mode.