A MODIFIED ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR MEASURING TYPE-SPECIFIC ANTI-PNEUMOCOCCAL CAPSULAR POLYSACCHARIDE ANTIBODIES

被引：53
|
作者：
KONRADSEN, HB
SORENSEN, UBS
HENRICHSEN, J
机构：
[1] Department of Bacteriology, Division of Diagnostic Microbiology, Statens Seruminstitut, Artillerivej 5
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1993年 / 164卷 / 01期
关键词：
ELISA; PNEUMOCOCCAL ANTIBODY; C-POLYSACCHARIDE ANTIBODY INHIBITION; IGG; (SUBCLASS);
D O I：
10.1016/0022-1759(93)90270-H
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed an ELISA for antibody determination, superior to others hitherto described, in which optimal coating is achieved using phenylated pneumococcal capsular polysaccharides as coating antigen. The specificity of the assay is ensured by complete inhibition of antibodies against the species-specific pneumococcal antigen, C-polysaccharide (C-Ps). The method is sensitive, specific, reproducible, fast and easy to work with and can be used for both immunoglobulin class and subclass antibody determinations.
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页码：13 / 20
页数：8
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