CLONING AND SEQUENCE-ANALYSIS OF DNA GYRASE GENES FROM MYCOBACTERIUM-TUBERCULOSIS

被引：0
|
作者：
MADHUSUDAN, K [1 ]
RAMESH, V [1 ]
NAGARAJA, V [1 ]
机构：
[1] INDIAN INST SCI, CTR GENET ENG, BANGALORE 560012, KARNATAKA, INDIA
来源：
CURRENT SCIENCE | 1994年 / 66卷 / 09期
关键词：
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Using a heterologous DNA gyrase probe from Streptomyces sphaeroides, we have cloned the gyrA and gyrB genes of Mycobacterium tuberculosis. The M. tuberculosis gyrB gene has been sequenced and compared with the amino acid sequences of GyrB from other bacteria. There is a significant level (60-80%) of amino acid sequence homology between GyrB of M. tuberculosis and that of other bacterial species, indicating a high degree of conservation of this essential enzyme.
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页码：664 / 667
页数：4
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