MONOCLONAL-ANTIBODY EPITOPES OF MYCOBACTERIAL 65-KD HEAT-SHOCK PROTEIN DEFINED BY EPITOPE SCANNING

被引：0
|
作者：
HAJEER, AH
WORTHINGTON, J
MORGAN, K
BERNSTEIN, RM
机构：
[1] UNIV MANCHESTER,SCH MED,DEPT RHEUMATOL,MANCHESTER M13 9PT,LANCS,ENGLAND
[2] UNIV MANCHESTER,SCH MED,ARC EPIDEMIOL UNIT,MANCHESTER M13 9PT,LANCS,ENGLAND
来源：
CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 1992年 / 89卷 / 01期
关键词：
B-CELL EPITOPES; MONOCLONAL ANTIBODIES; MYCOBACTERIAL HSP65; EPITOPE SCANNING;
D O I：
暂无
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The binding sites for MoAbs to the 65-kD heat-shock protein (hsp65) of mycobacteria have been investigated by epitope scanning. Five hundred and twenty-six 8-mer peptides representing the complete sequence of Mycobacterium tuberculosis hsp65 were synthesised in duplicate using the Epitope Scanning Kit (CRB Ltd.). The epitopes of six MoAbs raised to the hsp65 of M. tuberculosis or M. leprae were investigated. We have identified the epitope of a new MoAb (DC 16); this epitope is continuous, hydrophilic in nature and 11 amino acids long. We have also confirmed the location of the epitopes of three MoAbs (IIH9, ML30 and IIC8). Thus the epitope scanning technique has proved suitable for the detection of continuous epitopes of hsp65.
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页码：115 / 119
页数：5
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