A BETA-D-XYLOSIDASE FROM THE THERMOPHILE CALDOCELLUM-SACCHAROLYTICUM EXPRESSED IN ESCHERICHIA-COLI

被引：11
|
作者：
LUTHI, E [1 ]
BERGQUIST, PL [1 ]
机构：
[1] UNIV AUCKLAND, CTR GENE TECHNOL, DEPT CELLULAR & MOLEC BIOL, AUCKLAND, NEW ZEALAND
来源：
FEMS MICROBIOLOGY LETTERS | 1990年 / 67卷 / 03期
关键词：
Caldocellum saccharolyticum; Escherichia coli; Thermophile; β-d-Xylosidase;
D O I：
10.1016/0378-1097(90)90011-E
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The gene coding for a β-d-xylosidase (E.C. 3.2.1.37) of the thermophile Caldocellum saccharolyticum was isolated previously as part of a gene cluster which has been cloned in Escherichia coli. The enzyme characteristics were determined in E. coli using toluenized cell extracts. The pH optimum was 6.5 and temperature optimum 70°C. The enzyme was stable at 60°C and the half life at 80°C was 45 minutes. The temperature optimum and the temperature stability exceed those reported for other bacterial or fungal β-d-xylosidases. The enzyme showed no other detectable xylanolytic or cellulolytic enzyme activity. © 1990.
引用
收藏
页码：291 / 294
页数：4
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