PSEUDOMONAS SIDEROPHORE PYOCHELIN ENHANCES NEUTROPHIL-MEDIATED ENDOTHELIAL-CELL INJURY

Pyochelin, a siderophore secreted by Pseudomonas aeruginosa, binds iron in a form which can catalyze the formation of hydroxyl radical (.OH) from neutrophil-derived superoxide (O-2(-).) and hydrogen peroxide (H2O2) Ferripyochelin induced a concentration-dependent increase in endothelial cell injury (Cr-51 release) resulting from exposure to H2O2, a xanthine/xanthine oxidase O-2(-)./H2O2 generating system, or stimulated neutrophils. This process was dependent on the presence of iron. Formation of OH was confirmed using spin trapping. Although a slight (13%) increase in neutrophil O-2(-). production in the presence of ferripyochelin was observed, this did not appear to account for the extent of endothelial cell injury observed. The antioxidants dimethylthiourea and catalase decreased endothelial cell injury, whereas dimethyl sulfoxide and superoxide dismutase were without effect. Fe-nitrilotriacetic acid and Fe-EDTA, which are also OH catalysts, did not augment endothelial cell injury resulting from exposure to the above oxidant systems. In contrast to results with the endothelial cells, killing of P. aeruginosa by O-2(-)./H2O2 derived from the reaction of xanthine and xanthine oxidase was not increased by ferripyochelin. These data are consistent with the possibility that the interaction of Pseudomonas- and phagocyte-derived secretory products could contribute to local tissue injury at sites of P. aeruginosa infection by causing the generation of .OH.