A RAPID METHOD FOR STARTING A CULTURE FOR THE ESTABLISHMENT OF EPSTEIN-BARR VIRUS-TRANSFORMED HUMAN LYMPHOBLASTOID CELL-LINES

被引：24
|
作者：
FUKUSHIMA, Y
OHASHI, H
WAKUI, K
NISHIDA, T
OHISHI, T
机构：
[1] SAITAMA CHILDRENS MED CTR,DEPT CLIN LAB,IWATSUKI,SAITAMA 339,JAPAN
[2] SAITAMA CHILDRENS MED CTR,DIV INFECT DIS IMMUNOL & ALLERGOL,IWATSUKI,SAITAMA 339,JAPAN
来源：
JAPANESE JOURNAL OF HUMAN GENETICS | 1992年 / 37卷 / 02期
关键词：
CELL CULTURE; EB VIRUS-TRANSFORMED LYMPHOBLASTOID CELL LINES; A RAPID METHOD;
D O I：
10.1007/BF01899737
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We developed a simple and efficient procedure for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines. B-lymphocytes were obtained by centrifugation after hemolysis of red cells with a hemolysis buffer, instead of Ficoll-Parque gradient. We can start a primary culture within 15 min by using this method.
引用
收藏
页码：149 / 150
页数：2
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