OVEREXPRESSION OF CU-ZN SUPEROXIDE-DISMUTASE IN DROSOPHILA DOES NOT AFFECT LIFE-SPAN

被引：133
|
作者：
SETO, NOL
HAYASHI, S
TENER, GM
机构：
[1] Department of Biochemistry, University of British Columbia, Vancouver
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 11期
关键词：
Aging; Free radicals; P element transforatation; Paraquat; Transgenic Drosophila;
D O I：
10.1073/pnas.87.11.4270
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Aging and disease processes may be due to deleterious and irreversible changes produced by free radical reactions. The enzyme copper-zinc Superoxide dismutase (Cu-Zn SOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) performs a protective function by scavenging superoxide radicals. The Cu-Zn SOD gene (Sod) cloned from Drosophila melanogaster was introduced via P element-mediated transformation into the germ line. Homozygous lines carrying additional copies of the Sod gene were recovered and characterized. Increases in Sod transcripts and enzyme activity were observed in the transformed lines, indicating that all of the sequence information required for gene expression is contained on the inserted gene fragment. The effects of additional SOD on oxygen free radical metabolism and longevity were investigated. Additional SOD did not markedly affect oxygen metabolism or longevity.
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页码：4270 / 4274
页数：5
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