SINGLE-CHANNEL ANALYSIS OF A CLONED HUMAN HEART L-TYPE CA2+ CHANNEL ALPHA(1)-SUBUNIT AND THE EFFECTS OF A CARDIAC BETA-SUBUNIT

被引：45

作者：

WAKAMORI, M ^{[1
]}

MIKALA, G ^{[1
]}

SCHWARTZ, A ^{[1
]}

YATANI, A ^{[1
]}

机构：

[1] UNIV CINCINNATI,SCH MED,DEPT PHARMACOL & CELL BIOPHYS,CINCINNATI,OH 45221

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.2374

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Macroscopic and single-channel properties of a cloned human heart L-type Ca2+ channel α1 subunit expressed in Xenopus oocytes were studied and the effects of a cardiac β subunit were evaluated. The α1 subunit expressed current with much slower activation and inactivation kinetics than native cells. The β subunit increased the current amplitude and accelerated both activation and inactivation rates. Single-channel analysis revealed that the β subunit increased the probability of channel opening and shifted the voltage-dependence to more negative potentials without affecting conductance or open time. The data suggest that the β subunit modulates macroscopic current by increasing the probability of channel opening and shifting the voltage-dependence of the channel. © 1993 Academic Press. All rights reserved.

引用

页码：1170 / 1176

页数：7

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