PREPARATION AND CHARACTERIZATION OF ANTI-FUMONISIN MONOCLONAL-ANTIBODIES

被引：31
|
作者：
FUKUDA, S [1 ]
NAGAHARA, A [1 ]
KIKUCHI, M [1 ]
KUMAGAI, S [1 ]
机构：
[1] NATL INST HLTH, SHINJUKU KU, TOKYO 162, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 04期
关键词：
D O I：
10.1271/bbb.58.765
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Murine monoclonal antibodies were prepared against fumonisin B-1-carrier protein conjugates, such as fumonisin B-1-ovalbumin and fumonisin B-1-keyhole limpet hemocyanin. Five stable hybridoma clones were obtained. The response ranges of these monoclonal antibodies for fumonisin B-1 were from 10 to 1000-5000 ng/ml in the competitive indirect enzyme-linked immunosorbent assay (CI-ELISA).
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页码：765 / 767
页数：3
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