SOLID-PHASE SYNTHESIS AND HYBRIDIZATION PROPERTIES OF DNA CONTAINING SULFIDE-LINKED DINUCLEOSIDES

被引：33

作者：

KAWAI, SH ^{[1
]}

WANG, D ^{[1
]}

GIANNARIS, PA ^{[1
]}

DAMHA, MJ ^{[1
]}

JUST, G ^{[1
]}

机构：

[1] MCGILL UNIV, DEPT CHEM, MONTREAL H3A 2K6, QUEBEC, CANADA

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/nar/21.6.1473

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligodeoxyribonucleotides incorporating non-hydrolyzable dialkyl sulfide linked thymidine dimers (TsT) were synthesized chemically by the solid-phase approach. The sulfide dimer TsT was stable to degradation by snake-venom phosphodiesterase, calf spleen phosphodiesterase, Nuclease P1 and Nuclease S1. Thermal denaturation analysis indicated that the incorporation of TsT dimers into DNA weakened, but did not prevent, binding to complementary DNA and RNA over a wide range of salt concentrations (10 mM to 2 M NaCl).

引用

页码：1473 / 1479

页数：7

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