CROSSABILITY ELUCIDATION BETWEEN Saccharum spp. and Erianthus sp. ACCESSIONS USING SSR MARKER

Commercial sugarcane varieties, which are widely planted in Indonesia, originated from intraspecific and/or interspesific hybridization of Saccharum spp. However, hybridization in sugarcane is not always easy to do. The morphology of sugarcane flower and environment are two factors that influence the success of hybridization. The similarity of phenotypic appearance of progeny and parent is another limiting factor in indentifying the hybrid. The objective of this study was to identify the progeny from intra and interspecific hybridization as true hybrid, selfing, or off type using simple sequence repeat (SSR). This study was conducted in the Molecular Genetics laboratory of the Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) in Malang, Indonesia from August 2016 to July 2017. There were 91 genotypes consisting of four Saccharum spp. and one Erianthus sp. as parent and 86 F1 intra and interspecific progeny. Identification of putative hybrids was done by comparing the visualization band result from electrophoresis of male and female parent genetic marker in F1 hybrid. All primers could identify on average 62.7%, 52.44%, and 38.89% of the progenies as hybrid in crosses among sugarcane commercial varieties, between sugarcane commercial varieties and S. spontaneum, and between sugarcane commercial varieties and Erianthus sp. Further, primers also identified in average 8.02%, 30.21%, and 24.44% of the progenies as selfing from crosses among sugarcane commercial varieties, between sugarcane commercial varieties and S. spontaneum, and between sugarcane commercial varieties and Erianthus sp. Also, 29.42%, 17.46%, and 36.11% of the progenies could be identified as off type by primers from crosses among sugarcane commercial varieties, between sugarcane commercial varieties and between S. spontaneum, and crossing sugarcane commercial varieties and Erianthus sp.