Phytochemical Analysis and In-vitro Antioxidant and cytotoxic activity of Aerial parts of Cestrum aurantiacum and Solanum mauritianum (Solanaceae weeds of Niligiris)

To investigate the microscopical, Phytochemical (Qualitative and Quantitative) and In-vitro antioxidant studies of hydro alcohol (70%), Aqueous, Ethyl acetate, Chloroform, Pet - ether and total saponin fractions of aerial parts of Cestrum aurantiacum (C. aurantiacum) and Solanum mauritianum (S. mauritianum). The Phytochemical quantification of the total phenolic, flavanoid and alkaloid contents were estimated using gallic acid, rutin and atropine as standard; Total saponin content was isolated and estimated by using gravimetric analysis; In-vitro antioxidant activity was evaluated for extracts by using different free radicals (ABTS and DPPH) assay techniques. S. mauritianum aerial parts hydro-ethanol (70%) extract have more phenolic content, C. aurantiacum aerial parts hydro-ethanol extract have more of alkaloid content, flavanoid and saponin content. The total saponin fractions showed potent IC50 values of 55.69 ug/ml and 54.99 ug/ml for ABTS assay and IC50 values of 119.95 ug/ml and 101.68 ug/ml for DPPH assay. The cytotoxic assay was carried for the total saponins on MCF-7 and HCT-116 cell lines and showed potent cytotoxic activity of 212+/-0.23 ug/mL and 67+/-1.32 ug/mL on MCF-7 cell lines and on HCT-116 cell lines with 208+/-0.34 ug /mL and 308+/-0.47ug/mL. Conclusion: In present study, we found that the selected plants posses' good amount of phenolics, alkaloids, flavanoids and saponins, among all the fractions the total saponin fractions showed better antioxidant activity in compare to other fractions, The fractions also showed a good In-vitro cytotoxic activity on MCF-7 cell lines and moderate against HCT-116 cell lines in comparison to standard Salvicine