ANTIOXIDANT ACTIVITY OF AQUEOUS SEED EXTRACT OF ANETHUM GRAVEOLENS L.

To provide a scientific basis to traditional uses of seeds of Anethum graveolens, seed extract was evaluated for its antioxidative potential. Firstly, indeed, aqueous extract of Anethum graveolens was prepared and analyzed for the phytochemical compounds contents. Furthermore, antioxidant activity was assessed using the Total Antioxidant Capacity (TAC) methods: 2.2' Azinobis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS. +), 2, 2-Diphenyl-1-picrylhydrazyl (DPPH.+), Ferric reducing antioxidant power (FRAP) and also the Hydroxyl Radical Scavenging Activity (HRSA) assays. The total phenolic, catechins and tannins contents of this extract were also assessed. Finally, the protection effect on DNA damage was evaluated. Results obtained by all of the radical scavenging activity methods used showed higher antioxidant activity. Also, the results indicate that the extracts of A. graveolens showed stronger hydroxyl radical scavenging activity with an inhibition percentage of 43.56 %. However, results obtained showed that the extract was not providing a protective effect upon DNA fragmentation by the method used.