CLARIFICATION OF THE STRUCTURAL AND FUNCTIONAL FEATURES OF THE OSMOREGULATED KDP OPERON OF ESCHERICHIA-COLI

被引：63

作者：

SUGIURA, A ^{[1
]}

NAKASHIMA, K ^{[1
]}

TANAKA, K ^{[1
]}

MIZUNO, T ^{[1
]}

机构：

[1] NAGOYA UNIV, SCH AGR, MICROBIOL LAB, CHIKUSA KU, NAGOYA, AICHI 464, JAPAN

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 13期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01349.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of the Escherichia coll kdpABC operon, which is responsible for a high-affinity potassium-uptake system, is regulated in response to a change in the medium osmolarity. In this study, we clarified the structure and function of the kdpABC promoter including its regulatory sequence at the molecular level. The canonical -35 and -10 regions determined for the promoter were not fully functional, i.e. in addition to them, a cis-acting sequence located upstream of the -35 region was essential for full activation of the promoter. This upstream sequence was demonstrated to be the target site for the trans-acting activator, KdpE.

引用

页码：1769 / 1776

页数：8

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