PROTEIN-PHOSPHORYLATION BY INORGANIC PYROPHOSPHATE IN YEAST MITOCHONDRIA

被引：8
|
作者：
DASILVA, LP [1 ]
LINDAHL, M [1 ]
LUNDIN, M [1 ]
BALTSCHEFFSKY, H [1 ]
机构：
[1] UNIV STOCKHOLM,ARRHENIUS LAB,DEPT BIOCHEM,S-10691 STOCKHOLM,SWEDEN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
D O I：
10.1016/0006-291X(91)91043-C
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Inorganic pyrophosphate can function as phosphate donor in protein phosphorylation reactions in yeast mitochondria. It was shown that, when PPi substitutes for ATP as inhibitor of the pyruvate dehydrogenase reaction, maximal activity is reached after a lag-period of 30-60 minutes. 32P-labeling of peptides shows that [32P]PPi gives about 25% of the labeling obtained by [γ-32P]ATP in the protein kinase reaction. The PPi dependent phosphorylation is increased several fold by the presence of cold ATP. © 1991.
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页码：1359 / 1364
页数：6
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