PURIFICATION AND CHARACTERIZATION OF AN EXTRACELLULAR LIPASE OF GALACTOMYCES-GEOTRICHUM

被引：19
|
作者：
PHILLIPS, A
PRETORIUS, GHJ
机构：
[1] UNIV ORANGE FREE STATE, DEPT MICROBIOL & BIOCHEM, POB 339, BLOEMFONTEIN 9300, SOUTH AFRICA
[2] UNIV ORANGE FREE STATE, SASOL BIOTECHNOL CTR, BLOEMFONTEIN 9300, SOUTH AFRICA
来源：
BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 11期
关键词：
D O I：
10.1007/BF01026769
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Galactomyces geotrichum secretes an extracellular lipase with a relative molecular weight of 57 +/- 2 kDa in the unglycosylated state and 62 +/- 2 kDa when glycosylated. The enzyme has optimal activity at pH 7.75 and 30-degrees-C and has a pI of 5.2. The specificity of the lipase is similar to lipase A of Geotrichum candidum, but the amino acid composition of the lipases are different.
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页码：833 / 838
页数：6
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