TIME-RESOLVED CRYOELECTRON MICROSCOPIC STUDY OF THE DISSOCIATION OF ACTOMYOSIN INDUCED BY PHOTOLYSIS OF PHOTOLABILE NUCLEOTIDES

被引：57
|
作者：
MENETRET, JF
HOFMANN, W
SCHRODER, RR
RAPP, G
GOODY, RS
机构：
[1] MAX PLANCK INST MED RES,DEPT BIOPHYS,JAHNSTR 29,W-6900 HEIDELBERG,GERMANY
[2] EUROPEAN MOLEC BIOL LAB,OUTSTN,DEUTSCH ELEKTR SYNCHROTRON,W-2000 HAMBURG,GERMANY
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 219卷 / 02期
关键词：
TIME-RESOLVED CRYOELECTRON MICROSCOPY; CAGED NUCLEOTIDES; ACTOMYOSIN; ACTIN;
D O I：
10.1016/0022-2836(91)90554-J
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The rapid release of a substrate or other ligand from photolabile precursors in a thin layer suspension of biological specimens followed by rapid freezing provides a method of trapping and visualizing short-lived states in a dynamic system. We demonstrate here the first successful application of this method to study the interaction of actin filaments with myosin subfragment 1 (S1) after release of nucleotides. The results obtained suggest that structural changes in actin filaments occur as a result of interaction with S1. © 1991.
引用
收藏
页码：139 / 144
页数：6
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