MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF THE GENE ENCODING AN ENDO ALPHA-1,4 POLYGALACTOSAMINIDASE OF PSEUDOMONAS SP-881

被引：7
|
作者：
TAMURA, J [1 ]
HASEGAWA, K [1 ]
KADOWAKI, K [1 ]
IGARASHI, Y [1 ]
KODAMA, T [1 ]
机构：
[1] UNIV TOKYO,DEPT APPL BIOL CHEM,BUNKYO KU,TOKYO 113,JAPAN
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 80卷 / 04期
关键词：
ALPHA-1,4 POLYGALACTOSAMINIDASE; CHITINASE; PSEUDOMONAS; ALPHA-1,4 POLYGALACTOSAMINE;
D O I：
10.1016/0922-338X(95)94196-X
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The gene encoding an endo alpha-1,4 polygalactosaminidase (P-GalNase) from Pseudomonas sp. 881 was cloned into Escherichia coli DH1 and MV1184 by using charomid 9-36 and a pUC vector, and then sequenced. The gene encoding the enzyme P-GalNase consisted of 882 bp encoding 294 amino acid residues including a leader sequence. The deduced amino acid sequence did not show any extensive homology with sequences of other glycanases listed in the SWISS-PROT (GenBank) data bank, but we could observe some homology between the middle segment of the P-GalNase sequence and the conserved sequences of chitinases and related glycosidases.
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页码：305 / 310
页数：6
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