ISOLATION AND CELL-WALL REGENERATION OF PROTOPLASTS FROM BOTRYTIS-CINEREA PERS

被引：3
|
作者：
BRAUN, PJ
HEISLER, A
机构：
[1] Department of Biological Research, Agricultural Division, Hoechst AG, Frankfurt
来源：
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT | 1990年 / 128卷 / 04期
关键词：
D O I：
10.1111/j.1439-0434.1990.tb04277.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Abstract A procedure for efficient isolation and cell wall regeneration of protoplasts from Botrytis cinerea is described. Protoplasts were obtained from mycelia using a lytic enzyme mixture containing β‐Glucuronidase, Cellulase R10 and Driselase with mannitol for osmotic support. The digestion of cell walls was checked by fluorescence microscopy. Protoplasts were purified from cell debris and lytic enzymes. Regeneration and reversion were performed by incubation on agar plates. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：293 / 298
页数：6
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