Stimulation of TLRs, Nod-like receptors and Dectin-1 in neutrophils induces the production of proinflammatory cytokines

被引：0
|
作者：
Martinez Moreno, Jahnnyer Anilio [1 ]
Hernandez Lopez, Juan Carlos [2 ]
Urcuqui-Inchima, Silvio [1 ]
机构：
[1] Univ Antioquia, Fac Med, Grp Inmunovirol, Medellin, Colombia
[2] Univ Cooperativa Colombia, Fac Med, Medellin, Colombia
来源：
IATREIA | 2014年 / 27卷 / 02期
关键词：
C-type Lectin Receptors; Neutrophils; NOD-Like Receptors; Pattern Recognition Receptors; Pro-Inflammatory Cytokines; RIG-I-Like Receptors; Toll-Like Receptors;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Objective: To evaluate the expression and function of pattern recognition receptors such as Toll -like receptors, RIG-I/MDA5, NOD-like receptors, Dectin-1 and adaptor proteins, in human neutrophils. Methods: Neutrophils from peripheral blood of healthy individuals were purified and cultured in PPMI-1640, in the presence or absence of specific agonists of the receptor of interest. The expression of pattern recognition receptors was determined by PT-PCP and the secretion of proinflarnmatory cytoEi nes, by ELISA. Results: We observed that neutrophils express diverse patterns recognition receptors and adaptor molecules. Stimulation of TLP4, TLR5 and TLP7/8 induces the production of IL-1 beta and 1L-6, and activation of Dectin-1 leads to secretion of high levels of TNF-alpha, but low levels of IL-1 beta and IL-6. Conclusion: Neutrophils express a large number of pattern recognition receptors and their activation leads to the expression of proinflammatory cytokines.
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页码：135 / 146
页数：12
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