AN INVITRO METHOD FOR STUDYING ENDOCRINE AND EXOCRINE SECRETION IN THE PERFUSED ISOLATED RABBIT PANCREAS

被引：0
|
作者：
FICHAUX, F [1 ]
CATALA, J [1 ]
BONNAFOUS, R [1 ]
机构：
[1] UNIV TOULOUSE 3,INST PHYSIOL,PHYSIOL NUTR LAB,2 RUE FRANCOIS MAGENDIE,F-31400 TOULOUSE,FRANCE
来源：
DIABETES RESEARCH CLINICAL AND EXPERIMENTAL | 1990年 / 13卷 / 02期
关键词：
PERFUSED ISOLATED RABBIT PANCREAS; SURGICAL PREPARATION; INSULIN RELEASE; ALPHA-CHYMOTRYPSIN ACTIVITY;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
This work describes a perfusion technique adapted to the isolated rabbit pancreas allowing investigation of both the endocrine and exocrine function. The pancreas-duodenum-stomach-spleen complex is removed and perfused with a modified Krebs Ringer Bicarbonate medium. The surgical steps leading to the removal of the complex are described. The endocrine response is studied by measuring insulin release when the pancreas is submitted to successive glucose stimulations and the exocrine function is evaluated by the alpha-chymotrypsin activity of the pancreatic juice harvested during the perfusion.
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页码：95 / 100
页数：6
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