BINARY-LIQUID PHASE-SEPARATION OF LENS PROTEIN SOLUTIONS

被引:292
|
作者
BROIDE, ML
BERLAND, CR
PANDE, J
OGUN, OO
BENEDEK, GB
机构
[1] MIT,DEPT PHYS,CAMBRIDGE,MA 02139
[2] MIT,CTR MAT SCI & ENGN,CAMBRIDGE,MA 02139
关键词
GAMMA-CRYSTALLIN; COEXISTENCE CURVE; CRITICAL PHENOMENA; COLD CATARACT; HOMOLOGY;
D O I
10.1073/pnas.88.13.5660
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have determined the coexistence curves (plots of phase-separation temperature T versus protein concentration C) for aqueous solutions of purified calf lens proteins. The proteins studied, calf gamma-IIIa-, gamma-IIIb-, and gamma-IVa-crystallin, have very similar amino acid sequences and three-dimensional structures. Both ascending and descending limbs of the coexistence curves were measured. We find that the coexistence curves for each of these proteins and for gamma-II-crystallin can be fit, near the critical point, to the function /(C(c) - C)/C(c)/= A[(T(c) - T)/T(c)]beta, where beta = 0.325, C(c) is the critical protein concentration in mg/ml, T(c) is the critical temperature for phase separation in K, and A is a parameter that characterizes the width of the coexistence curve. We find that A and C(c) are approximately the same for all four coexistence curves (A = 2.6 +/- 0.1, C(c) = 289 +/- 20 mg/ml), but that T(c) is not the same. For gamma-II- and gamma-IIIb-crystallin, T(c) almost-equal-to 5-degrees-C, whereas for gamma-IIIa- and gamma-IVa-crystallin, T(c) almost-equal-to 38-degrees-C. By comparing the published protein sequences for calf, rat, and human gamma-crystallins, we postulate that a few key amino acid residues account for the division of gamma-crystallins into low-T(c) and high-T(c) groups.
引用
收藏
页码:5660 / 5664
页数:5
相关论文
共 50 条
  • [1] KINETICS OF PHASE-SEPARATION IN BINARY-LIQUID MIXTURES
    WENZEL, J
    LIMBACH, U
    BRESONIK, G
    SCHNEIDER, GM
    [J]. JOURNAL OF PHYSICAL CHEMISTRY, 1980, 84 (15): : 1991 - 1995
  • [2] DYNAMICS OF PHASE-SEPARATION IN BINARY-LIQUID MIXTURES
    KNOBLER, CM
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1980, 180 (AUG): : 115 - COLL
  • [3] BINARY-LIQUID PHASE-SEPARATION AND CRITICAL PHENOMENA IN A PROTEIN WATER SOLUTION
    THOMSON, JA
    SCHURTENBERGER, P
    THURSTON, GM
    BENEDEK, GB
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) : 7079 - 7083
  • [4] VARIATIONAL THEORY OF PHASE-SEPARATION IN BINARY-LIQUID MIXTURES
    FOILES, SM
    ASCHCROFT, NW
    [J]. JOURNAL OF CHEMICAL PHYSICS, 1981, 75 (07): : 3594 - 3598
  • [5] PHASE-SEPARATION OF A BINARY-LIQUID MIXTURE IN A POROUS-MEDIUM
    GOH, MC
    GOLDBURG, WI
    KNOBLER, CM
    [J]. PHYSICAL REVIEW LETTERS, 1987, 58 (10) : 1008 - 1011
  • [6] CRITICAL-POINT PHASE-SEPARATION IN BINARY-LIQUID MIXTURES
    BOWEN, LH
    BENEVIDES, ML
    HAWORTH, DT
    [J]. JOURNAL OF CHEMICAL EDUCATION, 1993, 70 (09) : 775 - 775
  • [7] LATE-STAGE PHASE-SEPARATION AND SEDIMENTATION IN A BINARY-LIQUID MIXTURE
    CAU, F
    LACELLE, S
    [J]. PHYSICAL REVIEW E, 1993, 47 (02): : 1429 - 1432
  • [8] ADSORPTION FROM BINARY-LIQUID MIXTURES NEAR THE PHASE-SEPARATION POINT
    KUKLIN, RN
    KRESHCHISINA, LT
    [J]. RUSSIAN JOURNAL OF ELECTROCHEMISTRY, 1994, 30 (07) : 815 - 820
  • [9] CONVECTIVE INSTABILITIES AND DYNAMIC STRUCTURES IN PHASE-SEPARATION PROCESSES OF BINARY-LIQUID MIXTURES
    DITTMANN, M
    SCHNEIDER, GM
    [J]. ZEITSCHRIFT FUR NATURFORSCHUNG SECTION A-A JOURNAL OF PHYSICAL SCIENCES, 1986, 41 (04): : 678 - 680
  • [10] A CHARACTERISTIC TEMPERATURE FOR THE VISIBILITY OF PHASE-SEPARATION IN A BINARY-LIQUID MIXTURE UNDER SHEAR
    FUKUHARA, K
    HAMANO, K
    KUWAHARA, N
    SENGERS, JV
    KRALL, AH
    [J]. PHYSICS LETTERS A, 1993, 176 (05) : 344 - 348