HYDROLYSIS OF BRANCHED CYCLODEXTRINS BY A CYCLODEXTRIN-HYDROLYZING ENZYME FROM BACILLUS-SPHAERICUS E-244

被引：3
|
作者：
OGUMA, T [1 ]
KIKUCHI, M [1 ]
MIZUSAWA, K [1 ]
机构：
[1] KIKKOMAN FOODS INC,DIV RES & DEV,NODA,CHIBA 278,JAPAN
来源：
FEBS LETTERS | 1991年 / 290卷 / 1-2期
关键词：
BRANCH; CYCLODEXTRIN; CYCLOMALTODEXTRINASE; HYDROLYSIS; MALTOOLIGOSACCHARIDE; BACILLUS-SPHAERICUS;
D O I：
10.1016/0014-5793(91)81214-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The action of a cyclodextrin-hydrolyzing enzyme from Bacillus sphaericus E-244 on branched alpha- and beta-cyclodextrins was investigated. Glucosyl-alpha-cyclodextrin (6-O-alpha-D-glucosylcyclomaltohexaose) and maltosyl-alpha-cyclodextrin (6-O-alpha-D-maltosylcyclomaltohexaose) were hydrolyzed to 6(3)-O-alpha-D-glucosylmaltohexaose and 6(3)-O-alpha-D-maltosylmaltohexaose, respectively. Glucosyl-beta-cyclodextrin (6-O-alpha-D-glucosylcyclomaltoheptaose) and maltosyl-beta-cyclodextrin (6-O-alpha-D-maltosylcyclomaltoheptaose) were also transformed mainly to 6(4)-O-alpha-D-glucosylmaltoheptaose and 6(4)-O-alpha-D-maltosylmaltoheptaose, respectively. These results suggest that the cyclodextrin-hydrolyzing enzyme cleaves branched alpha- and beta-cyclodextrins at an alpha-1,4 linkage which is located furthest from the branching point on the cyclodextrin ring.
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页码：13 / 16
页数：4
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