A SIMPLE AND RAPID MICRO-KJELDAHL METHOD FOR TOTAL NITROGEN ANALYSIS

被引：2
|
作者：
Guebel, D. V. [1 ]
Nudel, B. C. [1 ]
Giulietti, A. M. [1 ]
机构：
[1] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Biotecnol, RA-1113 Buenos Aires, DF, Argentina
来源：
BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 06期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A fast and simple micro-method for total nitrogen analysis in complex fermentation media is presented. Samples containing up to 50 ug nitrogen are digested in tubes with sodium selenite (in sulphuric-phosphoric mixture) plus hydrogen peroxide during one hour at 380 degrees C. Ammonium liberated is measured without extraction by Berthelot calorimetric reaction.
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页码：427 / 430
页数：4
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