FUNCTIONAL-CHARACTERIZATION AND TRANSCRIPTIONAL ANALYSIS OF A GENE-CLUSTER GOVERNING EARLY AND LATE STEPS IN DAUNORUBICIN BIOSYNTHESIS IN STREPTOMYCES-PEUCETIUS

被引：61

作者：

MADDURI, K

HUTCHINSON, CR

机构：

[1] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 13期

关键词：

D O I：

10.1128/jb.177.13.3879-3884.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sequence analysis of the Streptomyces peucetius daunorubicin biosynthetic gene cluster revealed a partial (dnrQ) and two complete (dnrD and dnrP) open reading frames flanking dnrK. Bioconversion experiments showed that DnrD converts aklanonic acid methylester to aklaviketone and that DnrC is a methyltransferase that converts aklanonic acid to aklanonic acid methylester. The deduced dnrP gene product, homologous to known esterases, may catalyze the conversion of 10-carbomethoxy-13-deoxycarminomycin to its 10-carboxy derivative. The dnrKPQS genes may be transcribed as a polycistronic mRNA.

引用

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页码：3879 / 3884

页数：6

共 27 条

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MADDURI, K
HUTCHINSON, CR
JOURNAL OF BACTERIOLOGY, 1995, 177 (05) : 1208 - 1215
[2] The Streptomyces peucetius dpsY and dnrX genes govern early and late steps of daunorubicin and doxorubicin biosynthesis
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Hutchinson, CR
JOURNAL OF BACTERIOLOGY, 1998, 180 (09) : 2379 - 2386
[3] CLONING AND CHARACTERIZATION OF THE STREPTOMYCES-PEUCETIUS DNRQS GENES ENCODING A DAUNOSAMINE BIOSYNTHESIS ENZYME AND A GLYCOSYL TRANSFERASE INVOLVED IN DAUNORUBICIN BIOSYNTHESIS
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LIU, XC
FERGUSON, J
HUTCHINSON, CR
JOURNAL OF BACTERIOLOGY, 1995, 177 (22) : 6688 - 6692
[4] TRANSCRIPTIONAL ANALYSIS OF THE STREPTOMYCES-GLAUCESCENS TETRACENOMYCIN-C BIOSYNTHESIS GENE-CLUSTER
DECKER, H
HUTCHINSON, CR
JOURNAL OF BACTERIOLOGY, 1993, 175 (12) : 3887 - 3892
[5] Purification and characterization of the DNA-binding protein Dnrl, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius
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MOLECULAR MICROBIOLOGY, 1996, 22 (05) : 801 - 813
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LORY, S
INFECTION AND IMMUNITY, 1993, 61 (04) : 1371 - 1377
[7] GENE-CLUSTER FOR STREPTOMYCIN BIOSYNTHESIS IN STREPTOMYCES-GRISEUS - NUCLEOTIDE-SEQUENCE OF 3 GENES AND ANALYSIS OF TRANSCRIPTIONAL ACTIVITY
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NUCLEIC ACIDS RESEARCH, 1987, 15 (19) : 8041 - 8056
[8] GENE-CLUSTER FOR STREPTOMYCIN BIOSYNTHESIS IN STREPTOMYCES-GRISEUS - ANALYSIS OF A CENTRAL REGION INCLUDING THE MAJOR RESISTANCE GENE
DISTLER, J
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MOLECULAR & GENERAL GENETICS, 1987, 208 (1-2): : 204 - 210
[9] CHARACTERIZATION AND TRANSCRIPTIONAL ANALYSIS OF THE GENE-CLUSTER FOR CORONAFACIC ACID, THE POLYKETIDE COMPONENT OF THE PHYTOTOXIN CORONATINE
LIYANAGE, H
PALMER, DA
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APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (11) : 3843 - 3848
[10] FURTHER CHARACTERIZATION OF THE HISTIDINE GENE-CLUSTER OF STREPTOMYCES-COELICOLOR A3(2) - NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL ANALYSIS OF HISD
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BRUNI, CB
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