ETHANE PRODUCTION-RATE INVIVO IS REDUCED WITH DIETARY RESTRICTION

Dietary restriction without malnutrition prolongs life and has a beneficial effect on age-related diseases and metabolic derangements. To test th eeffect of food restriction on ethane production rate, ethane exhalation was measured in rats with partial food restriction. Ethane production rate in room air in rats fed 60% of food consumed by ad libitum-fed animals for 2 wk was significantly reduced (3.50 ± 0.25 vs. 5.21 ± 0.34 pmol · min-1 · 100 g body wt-1, P < 0.01). In 100% oxygen, ethane production in food-restricted rats was not different from that of ad libitum-fed rats (21.81 ± 1.25 vs. 19.57 ± 1.89 pmol · min-1 · 100 g-1). Fifteen hours of fasting compared with ad libitum feeding reduced ethane production modestly in room air (4.37 ± 0.45 vs. 5.21 ± 0.34 pmol · min-1 · 100 g-1) and more significantly in 100% oxygen (12.37 ± 0.78 vs. 19.57 ± 1.89 pmol · min-1 · 100 g-1). Thus, in 100% oxygen, 15 h of fasting, compared with ad libitum feeding, resulted in an ~40% decrease in ethane production rate. It is concluded that short-term food restriction significantly reduces ethane exhalation rate in rats when measured in room air.