RFLP TAGGING OF A GENE PM12 FOR POWDERY MILDEW RESISTANCE IN WHEAT (TRITICUM-AESTIVUM L)

A dominant gene, Pm12, conferring the resistance to powdery mildew (Erysiphe graminis) has been transferred to wheat from Aegilops speltoides. Sixteen RFLP probes were used to test Line 31 and its parents, showing that Line 31 is a 6B/6S translocation lines. The linkage analysis by five probes indicates that Pm12 is located on the chromosome 6B/6S, and tightly flanked by an RFLP marker alpha-Amy-1 (1.1 cM), identified by RFLP and isozyme analysis. This approach is equally applicable to the identification of other transferred alien genes and the segments of alien chromosome introgressed into wheat.