IN-VITRO EFFECTS OF PROSTAGLANDIN-E1 AND INDOMETHACIN ON MITOMYCIN C-INDUCED SISTER-CHROMATID EXCHANGES IN MITOGEN-STIMULATED HUMAN-LYMPHOCYTES

被引：6
|
作者：
EKMEKCI, A
SAYLI, A
DONMEZ, H
BAL, F
机构：
[1] Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, 06510 Besevler, Ankara
来源：
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1995年 / 328卷 / 01期
关键词：
MITOMYCIN C; PROSTAGLANDIN E1; INDOMETHACIN; SISTER-CHROMATID EXCHANGE; HUMAN LYMPHOCYTES;
D O I：
10.1016/0027-5107(94)00196-C
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In this study, the individual and combined effects of prostaglandin E1 (PGE1) and Indomethacin on mitomycin C (MMC)-induced SCEs in human lymphocytes was investigated in vitro. All MMC-treated cultures showed a great increase of SCEs (approximately two-fold), indicating its ability to induce mutations. SCE data showed that MMC-induced SCEs were reduced significantly in the presence of PGE1 in pooled analysis of six experiments (60.55% reduction of SCEs at 10(-6) M, 34.13% reduction of SCEs at 10(-9) M). In contrast the presence of indomethacin in the medium during MMC treatment of cells failed to show a significant reduction of SCEs in pooled analysis (21.17%). However, individual analyses revealed only two of six donors with a significant SCE response. Thus, the findings suggest that PGE1 can modify the DNA damaging effect of carcinogens and thereby may prevent the initiation of the carcinogenic process.
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页码：49 / 53
页数：5
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