IMAGING GLUCOAMYLASE BY SCANNING-TUNNELING-MICROSCOPY

被引：7
|
作者：
GUNNING, AP
MORRIS, VJ
WILLIAMSON, G
BELSHAW, NJ
KRAMER, GFH
KANNING, MW
机构：
[1] INST FOOD RES, NORWICH LAB, NORWICH NR4 7UA, NORFOLK, ENGLAND
[2] AGR UNIV WAGENINGEN, DEPT FOOD CHEM & MICROBIOL, WAGENINGEN, NETHERLANDS
来源：
ANALYST | 1994年 / 119卷 / 09期
关键词：
SCANNING TUNNELING MICROSCOPY; ATOMIC FORCE MICROSCOPY; SCANNING PROBE MICROSCOPY; GLUCOAMYLASE; BIOMOLECULE;
D O I：
10.1039/an9941901939
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.
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页码：1939 / 1942
页数：4
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