SELECTIVE CHEMICAL AUTOLIGATION ON A DOUBLE-STRANDED DNA-TEMPLATE

被引：26
|
作者：
HERRLEIN, MK [1 ]
LETSINGER, RL [1 ]
机构：
[1] NORTHWESTERN UNIV,DEPT CHEM,EVANSTON,IL 60208
来源：
NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 23期
关键词：
D O I：
10.1093/nar/22.23.5076
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We show that a double-stranded DNA segment serves as an effective template for spontaneously coupling short pyrimidine oligonucleotides containing terminal -P(O)(O-S- and BrCH2C(O)NH- groups. The efficiency of this autoligation depends markedly on proper base-pairing between the probe oligomers and the double-stranded target. This chemistry should be useful in designing highly selective probes for double-stranded polynucleotide segments.
引用
收藏
页码：5076 / 5078
页数：3
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